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tgf β type i receptor tβri inhibitors sd208  (Tocris)


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    Tocris tgf β type i receptor tβri inhibitors sd208
    (A) Dot plot showing Ephb2 gene expression across different hepatic cell types in mouse snRNA-seq analyzed from published dataset GSE212837. (B) Representative immunofluorescence images of human liver sections of healthy and MASH patients stained with antibodies against phosphoEphB2 (Red) and αSMA (green), DAPI counterstain for nuclei is shown in blue. (C) Messenger RNA level of EPHB2 , COL1A2 , and ACTA2 in TGF-β1 stimulated primary human HSCs at various time point (n=3). (D) Western blot showing proteins level of EphB2, aSMA, pSmad2/3 Smad2/3 and Vinculin in TGF-β1 stimulated primary human HSCs at various time point. (E) Messenger RNA level of EPHB2 upon inhibition of canonical TGF-β/SMAD with SIS3 Smad3 inhibitor, <t>SD208</t> TGFβR1 inhibitor, and SB525334 TGFβR2 inhibitor in TGF-β1-treated primary human HSCs (n=5-7). (F) Effects of silencing Smad2 and Smad3 with Mission®-esiRNA on TGF-β-induced EPHB2 mRNA expression in primary human HSCs (n=3). Non-targeting Mission®-esiRNA was used as control. (G) Accessibility peaks for the transcription factor SMAD3 on the promoter of human EPHB2 locus identified from a publicly available ChIP-seq dataset of TGF-β1 stimulated LX2 cells (GSE38103). (H) Enrichment of SMAD3 binding sites on the promoter of human EPHB2 validated by ChIP-qPCR in TGF-β1 stimulated primary human HSCs. All these data presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by ANOVA ns = not significant.
    Tgf β Type I Receptor Tβri Inhibitors Sd208, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tgf+%CE%B2+type+i+receptor+t%CE%B2ri+inhibitors+sd208/SD+208/bio_rxiv__2025__11__19__688968-231-16-34
    Average 94 stars, based on 69 article reviews
    tgf β type i receptor tβri inhibitors sd208 - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "EPHB2 promotes diet-induced MASH liver fibrosis"

    Article Title: EPHB2 promotes diet-induced MASH liver fibrosis

    Journal: bioRxiv

    doi: 10.1101/2025.11.19.688968

    (A) Dot plot showing Ephb2 gene expression across different hepatic cell types in mouse snRNA-seq analyzed from published dataset GSE212837. (B) Representative immunofluorescence images of human liver sections of healthy and MASH patients stained with antibodies against phosphoEphB2 (Red) and αSMA (green), DAPI counterstain for nuclei is shown in blue. (C) Messenger RNA level of EPHB2 , COL1A2 , and ACTA2 in TGF-β1 stimulated primary human HSCs at various time point (n=3). (D) Western blot showing proteins level of EphB2, aSMA, pSmad2/3 Smad2/3 and Vinculin in TGF-β1 stimulated primary human HSCs at various time point. (E) Messenger RNA level of EPHB2 upon inhibition of canonical TGF-β/SMAD with SIS3 Smad3 inhibitor, SD208 TGFβR1 inhibitor, and SB525334 TGFβR2 inhibitor in TGF-β1-treated primary human HSCs (n=5-7). (F) Effects of silencing Smad2 and Smad3 with Mission®-esiRNA on TGF-β-induced EPHB2 mRNA expression in primary human HSCs (n=3). Non-targeting Mission®-esiRNA was used as control. (G) Accessibility peaks for the transcription factor SMAD3 on the promoter of human EPHB2 locus identified from a publicly available ChIP-seq dataset of TGF-β1 stimulated LX2 cells (GSE38103). (H) Enrichment of SMAD3 binding sites on the promoter of human EPHB2 validated by ChIP-qPCR in TGF-β1 stimulated primary human HSCs. All these data presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by ANOVA ns = not significant.
    Figure Legend Snippet: (A) Dot plot showing Ephb2 gene expression across different hepatic cell types in mouse snRNA-seq analyzed from published dataset GSE212837. (B) Representative immunofluorescence images of human liver sections of healthy and MASH patients stained with antibodies against phosphoEphB2 (Red) and αSMA (green), DAPI counterstain for nuclei is shown in blue. (C) Messenger RNA level of EPHB2 , COL1A2 , and ACTA2 in TGF-β1 stimulated primary human HSCs at various time point (n=3). (D) Western blot showing proteins level of EphB2, aSMA, pSmad2/3 Smad2/3 and Vinculin in TGF-β1 stimulated primary human HSCs at various time point. (E) Messenger RNA level of EPHB2 upon inhibition of canonical TGF-β/SMAD with SIS3 Smad3 inhibitor, SD208 TGFβR1 inhibitor, and SB525334 TGFβR2 inhibitor in TGF-β1-treated primary human HSCs (n=5-7). (F) Effects of silencing Smad2 and Smad3 with Mission®-esiRNA on TGF-β-induced EPHB2 mRNA expression in primary human HSCs (n=3). Non-targeting Mission®-esiRNA was used as control. (G) Accessibility peaks for the transcription factor SMAD3 on the promoter of human EPHB2 locus identified from a publicly available ChIP-seq dataset of TGF-β1 stimulated LX2 cells (GSE38103). (H) Enrichment of SMAD3 binding sites on the promoter of human EPHB2 validated by ChIP-qPCR in TGF-β1 stimulated primary human HSCs. All these data presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by ANOVA ns = not significant.

    Techniques Used: Gene Expression, Immunofluorescence, Staining, Western Blot, Inhibition, esiRNA, Expressing, Control, ChIP-sequencing, Binding Assay, ChIP-qPCR

    Related Articles

    Expressing:

    Article Title: EPHB2 promotes diet-induced MASH liver fibrosis
    Article Snippet: .. To assess the effect of TGF-β/SMAD canonical pathway on EphB2 expression, HSCs were incubated with the TGF-β type I receptor (TβRI) inhibitors SD208 (1μM), SB525334 (200nM), and the SMAD3 inhibitor SIS3 (5μM) (all from Tocris) for 4h before stimulation with recombinant human TGF-β1 (10ng/ml) for 48h. ..

    Incubation:

    Article Title: EPHB2 promotes diet-induced MASH liver fibrosis
    Article Snippet: .. To assess the effect of TGF-β/SMAD canonical pathway on EphB2 expression, HSCs were incubated with the TGF-β type I receptor (TβRI) inhibitors SD208 (1μM), SB525334 (200nM), and the SMAD3 inhibitor SIS3 (5μM) (all from Tocris) for 4h before stimulation with recombinant human TGF-β1 (10ng/ml) for 48h. ..

    Recombinant:

    Article Title: EPHB2 promotes diet-induced MASH liver fibrosis
    Article Snippet: .. To assess the effect of TGF-β/SMAD canonical pathway on EphB2 expression, HSCs were incubated with the TGF-β type I receptor (TβRI) inhibitors SD208 (1μM), SB525334 (200nM), and the SMAD3 inhibitor SIS3 (5μM) (all from Tocris) for 4h before stimulation with recombinant human TGF-β1 (10ng/ml) for 48h. ..



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    tgf β type i receptor tβri inhibitors sd208  (Tocris)
    94
    Tocris tgf β type i receptor tβri inhibitors sd208
    (A) Dot plot showing Ephb2 gene expression across different hepatic cell types in mouse snRNA-seq analyzed from published dataset GSE212837. (B) Representative immunofluorescence images of human liver sections of healthy and MASH patients stained with antibodies against phosphoEphB2 (Red) and αSMA (green), DAPI counterstain for nuclei is shown in blue. (C) Messenger RNA level of EPHB2 , COL1A2 , and ACTA2 in TGF-β1 stimulated primary human HSCs at various time point (n=3). (D) Western blot showing proteins level of EphB2, aSMA, pSmad2/3 Smad2/3 and Vinculin in TGF-β1 stimulated primary human HSCs at various time point. (E) Messenger RNA level of EPHB2 upon inhibition of canonical TGF-β/SMAD with SIS3 Smad3 inhibitor, <t>SD208</t> TGFβR1 inhibitor, and SB525334 TGFβR2 inhibitor in TGF-β1-treated primary human HSCs (n=5-7). (F) Effects of silencing Smad2 and Smad3 with Mission®-esiRNA on TGF-β-induced EPHB2 mRNA expression in primary human HSCs (n=3). Non-targeting Mission®-esiRNA was used as control. (G) Accessibility peaks for the transcription factor SMAD3 on the promoter of human EPHB2 locus identified from a publicly available ChIP-seq dataset of TGF-β1 stimulated LX2 cells (GSE38103). (H) Enrichment of SMAD3 binding sites on the promoter of human EPHB2 validated by ChIP-qPCR in TGF-β1 stimulated primary human HSCs. All these data presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by ANOVA ns = not significant.
    Tgf β Type I Receptor Tβri Inhibitors Sd208, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tgf+%CE%B2+type+i+receptor+t%CE%B2ri+inhibitors+sd208/SD+208/bio_rxiv__2025__11__19__688968-231-16-34
    Average 94 stars, based on 1 article reviews
    tgf β type i receptor tβri inhibitors sd208 - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Dot plot showing Ephb2 gene expression across different hepatic cell types in mouse snRNA-seq analyzed from published dataset GSE212837. (B) Representative immunofluorescence images of human liver sections of healthy and MASH patients stained with antibodies against phosphoEphB2 (Red) and αSMA (green), DAPI counterstain for nuclei is shown in blue. (C) Messenger RNA level of EPHB2 , COL1A2 , and ACTA2 in TGF-β1 stimulated primary human HSCs at various time point (n=3). (D) Western blot showing proteins level of EphB2, aSMA, pSmad2/3 Smad2/3 and Vinculin in TGF-β1 stimulated primary human HSCs at various time point. (E) Messenger RNA level of EPHB2 upon inhibition of canonical TGF-β/SMAD with SIS3 Smad3 inhibitor, SD208 TGFβR1 inhibitor, and SB525334 TGFβR2 inhibitor in TGF-β1-treated primary human HSCs (n=5-7). (F) Effects of silencing Smad2 and Smad3 with Mission®-esiRNA on TGF-β-induced EPHB2 mRNA expression in primary human HSCs (n=3). Non-targeting Mission®-esiRNA was used as control. (G) Accessibility peaks for the transcription factor SMAD3 on the promoter of human EPHB2 locus identified from a publicly available ChIP-seq dataset of TGF-β1 stimulated LX2 cells (GSE38103). (H) Enrichment of SMAD3 binding sites on the promoter of human EPHB2 validated by ChIP-qPCR in TGF-β1 stimulated primary human HSCs. All these data presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by ANOVA ns = not significant.

    Journal: bioRxiv

    Article Title: EPHB2 promotes diet-induced MASH liver fibrosis

    doi: 10.1101/2025.11.19.688968

    Figure Lengend Snippet: (A) Dot plot showing Ephb2 gene expression across different hepatic cell types in mouse snRNA-seq analyzed from published dataset GSE212837. (B) Representative immunofluorescence images of human liver sections of healthy and MASH patients stained with antibodies against phosphoEphB2 (Red) and αSMA (green), DAPI counterstain for nuclei is shown in blue. (C) Messenger RNA level of EPHB2 , COL1A2 , and ACTA2 in TGF-β1 stimulated primary human HSCs at various time point (n=3). (D) Western blot showing proteins level of EphB2, aSMA, pSmad2/3 Smad2/3 and Vinculin in TGF-β1 stimulated primary human HSCs at various time point. (E) Messenger RNA level of EPHB2 upon inhibition of canonical TGF-β/SMAD with SIS3 Smad3 inhibitor, SD208 TGFβR1 inhibitor, and SB525334 TGFβR2 inhibitor in TGF-β1-treated primary human HSCs (n=5-7). (F) Effects of silencing Smad2 and Smad3 with Mission®-esiRNA on TGF-β-induced EPHB2 mRNA expression in primary human HSCs (n=3). Non-targeting Mission®-esiRNA was used as control. (G) Accessibility peaks for the transcription factor SMAD3 on the promoter of human EPHB2 locus identified from a publicly available ChIP-seq dataset of TGF-β1 stimulated LX2 cells (GSE38103). (H) Enrichment of SMAD3 binding sites on the promoter of human EPHB2 validated by ChIP-qPCR in TGF-β1 stimulated primary human HSCs. All these data presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by ANOVA ns = not significant.

    Article Snippet: To assess the effect of TGF-β/SMAD canonical pathway on EphB2 expression, HSCs were incubated with the TGF-β type I receptor (TβRI) inhibitors SD208 (1μM), SB525334 (200nM), and the SMAD3 inhibitor SIS3 (5μM) (all from Tocris) for 4h before stimulation with recombinant human TGF-β1 (10ng/ml) for 48h.

    Techniques: Gene Expression, Immunofluorescence, Staining, Western Blot, Inhibition, esiRNA, Expressing, Control, ChIP-sequencing, Binding Assay, ChIP-qPCR